9/6/2023 0 Comments Exclusion filters ultrasearchPolymer materials for the GFC are mostly based on polymethacrylate or polyhydroxymethacrylate. In order to reduce adsorption or other undesired interactions between the analyte and the stationary phase, the materials are specifically modified or undergo a special treatment during the manufacturing process. In many cases, suitable pore widths can be determined easily with the useful calibration data of the manufacturers.Īs the stationary phase either porous silica or polymer-based materials are used. These are often called "linear", "mixed gel" or "multi-pore gel". For samples with very different molar masses, either several columns of different pore sizes can be connected in series or columns containing particles of different pore sizes are used. The absolute pore sizes range from about 25 to 30.000 Å, covering a molecular weight range from about 100 to > 20.000.000 Daltons. The molecular weight of the analyte determines the pore size of the stationary phase. What is the molecular weight of my analyte? For non-polar polymers styrene-divinylbenzene copolymers (PS/DVB) usually serve as stationary phases and purely organic eluents are used.Ģ. The base material is often polymethacrylate or polyvinyl alcohol. Aqueous or polar organic eluents or mixtures of both are used for polar to medium-polar polymers. Silica gel has proven to be a suitable base material for this. How polar is the analyte and which solvents are suitable for itĪqueous buffer solutions are often used as eluents for very polar biomolecules, such as proteins or antibodies. For the selection of a suitable column, some basic questions should first be clarified.ġ.
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